|Hu, Pingsha - IOWA STATE UNIVERSITY|
Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: January 10, 2004
Publication Date: January 10, 2004
Citation: Hu, P., Wise, R.P. 2004. Physical mapping of new Lrk and Tak receptor-like kinase gene clusters in barley. Plant and Animal Genome Conference Proceedings. XII Conference. p. 857. Technical Abstract: Genes involved in resistance to pathogens are an intricate network of cell signaling events. A genome-wide search was undertaken to identify gene-rich BAC clones from barley that contained defense-related genes. HarvEST Triticeae (http://harvest.ucr.edu/) was utilized to identify ESTs uniquely associated with pathogen challenge. Of the 340,000 sequences in HarvEST, 194 ESTs were selected to derive overgo probes for hybridization to the barley (cv. Morex) BAC library. 3,662 hybridizing clones at a 1x genome equivalent of 3 BACs/overgo were identified. The identified BACs were estimated to cover 1.13% of the barley genome. Among the 3,662 clones, 108 addresses hybridized to more than one overgo pool. These 108 clones were further examined for physically linked defense-related gene clusters. Thirty-two BACs, which belong to three different linkage groups, were identified that contain Lrk and Tak receptor-like kinase gene clusters. Five new Lrk and eight Tak gene fragments were cloned via PCR from representative BACs from each linkage group. Our data suggest that overgo-based physical mapping of the barley gene-rich space is a highly efficient way for understanding the distribution of pathogen response genes, discovering gene clusters and facilitating gene cloning.