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United States Department of Agriculture

Agricultural Research Service

Title: Preclinical Diagnosis of African Swine Fever in Contact Exposed Swine by a Portable Real-Time Pcr Assay

Authors
item Zsak, Laszlo
item Borca, Manuel
item Risatti, Guillermo
item French, Richard - UNIVERSITY OF CONNECTICUT
item Lu, Zhiqiang
item Kutish, Gerald
item Neilan, John
item Callahan, Johnny - TETRACORE INC., MARYLAND
item Nelson, William - TETRACORE INC., MARYLAND
item Rock, Daniel

Submitted to: Conference Research Workers Disease Meeting
Publication Type: Proceedings
Publication Acceptance Date: November 16, 2004
Publication Date: November 16, 2004
Citation: Zsak, L., Borca, M.V., Risatti, G.R., French, R.A., Lu, Z., Kutish, G.F., Neilan, J.G., Callahan, J.D., Nelson, W.M., Rock, D.L. 2004. Preclinical diagnosis of african swine fever in contact exposed swine by a portable real-time pcr assay [abstract]. Conference Research Workers Disease Meeting. p. 87.

Technical Abstract: Clinical signs of African swine fever (ASF) are inapparent at early stages of infection and at later stages they resemble those of some other swine diseases. A rapid, preclinical diagnosis at the site of the suspected disease outbreak would be extremely useful for controlling ASF. To address this need, a fluorogenic probe hydrolysis (TaqMan®) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one step-single tube assay, which can be performed in two hours or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.

Last Modified: 8/29/2014
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