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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #167957
Title: BLACKBERRY YELLOW VEIN ASSOCIATED VIRUS: A NEW CRINIVIRUS FOUND IN BLACKBERRY

Author
item Martin, Robert
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item GERGERICH, R - UNIVERSITY OF ARKANSAS
item FERNANDEZ, G - NCSU DEPT OF HORTICULTURE
item PESIC, Z - NCSU DEPT OF PLANT PATHOL

Submitted to: Acta Horticulturae
Publication Type: Proceedings
Publication Acceptance Date: 5/13/2004
Publication Date: 9/25/2004
Citation: Martin, R.R., Tzanetakis, I.E., Gergerich, R.C., Fernandez, G., Pesic, Z. 2004. Blackberry yellow vein associated virus: a new crinivirus found in blackberry. Acta Horticulturae. 656:156-164.

Interpretive Summary: During the last three years, blackberries in southeastern U.S. have shown symptoms of vein clearing, yellow mottling, and plant decline with considerable variation in symptoms with cultivars. We isolated dsRNA from symptomatic plants and identified high molecular weight bands similar to those isolated from plants infected with criniviruses. Using degenerate primers developed against the crinivirus 1b protein in RT-PCR resulted in an amplicon that when sequenced showed the virus was a member of the Crinivirus genus. We have also cloned the virus and sequenced clones containing regions of the minor coat protein of the virus. Phylogenetic analysis revealed that the new virus, designated as Blackberry yellow vein associated virus (BYVaV), is most closely related to Beet pseudoyellows virus and Strawberry pallidosis associated virus, two criniviruses recently identified in strawberry.

Technical Abstract: During the last three years, blackberries in southern and southeastern USA have shown symptoms of vein clearing, yellow mottling, ringspots, and decline with considerable variation in symptoms among cultivars. We isolated dsRNA from symptomatic plants and identified high molecular weight bands similar to those isolated from plants infected with criniviruses. Paired extractions from virus tested blackberries did not yield any dsRNA bands with molecular weight greater than 500 bp. Using degenerate primers developed against the crinivirus polymerase in RT-PCR resulted in an amplicon that when sequenced showed the virus was a member of the Crinivirus genus. We have also cloned the virus and sequenced clones containing regions of the minor coat protein of the virus. Phylogenetic analysis revealed that the new virus, designated here as Blackberry yellow vein associated virus (BYVV), is most closely related to the Beet pseudo yellows virus and Strawberry pallidosis associated virus, two criniviruses recently identified in strawberry.