Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2004
Publication Date: July 11, 2004
Citation: Horvath, D.P., Chao, W.S. 2004. At1g36390 is highly conserved, and may play a role in shoot development [Abstract]. 15th International Conference on Arabidopsis Research. Page No. T02-047.
Abstract:At1g63690 was identified as a gene expressed preferentially in the shoots of leafy spurge (Euphorbia esula) as shown by hybridizing labeled shoot vs leaf cDNA populations from leafy spurge to Arabidopsis cDNA arrays. Subsequent expression analysis of this gene demonstrated that it was expressed preferentially in actively growing shoot apices of leafy spurge. A search of the various DNA sequence databases identified putative orthologues of this gene in all plant species examined, and similar genes of unknown function in mammals, yeast, insects, and roundworms. Sequence analysis of the gene indicated that it encodes a protein with a growth factor receptor signature. The encoded protein contains a putative leader sequence, PA domain (Protease Associated), and a series of trans-membrane domains through the carboxy terminal half of the protein, suggesting it may play a role in the signal transduction processes. Arabidopsis plants putatively containing insertion mutations in this gene display the formation of aerial rosettes and/or a very late flowering phenotype. Tobacco plants transformed with RNAi constructs designed to knock out expression of the orthologous gene display a rolled and wavy leaf phenotype. Combined, these results suggest that At1g63690 encodes a previously uncharacterized protein involved in meristem development.