Technical Abstract: Vegetatively-propagated mint plants can be successfully cryopreserved when they are adequately dehydrated and treated with cryoprotectants. Many of these treatments are known to be necessary, yet damaging. The extent of plasmolysis and the effects of these treatments on plasmodesmata have not been reported in the literature. First, we ensured that significant levels of deplasmolysis did not occur during fixation and embedding procedures. We then quantified the extent of plasmolysis during each step of the cryopreservation procedure. In the meristem, the 0.3 M sucrose, 2M glycerol/0.6 M sucrose for 20 minutes, and the PVS2 treatments did not plasmolyze the cells. The plasmodesmata within the shoot tips appeared to be intact after cells were treated with 0.3 M sucrose for 2 hours. Cells were significantly plasmolyzed after treatment with 1.2 M sucrose for 20 minutes during recovery after liquid nitrogen treatment. Some cytoplasm remained associated with the plasmodesmata when plasmolysis occurred. When cells were treated with 1.2 M sucrose, followed by 0.3 M sucrose prior to fixation and embedding, deplasmolysis occurred. These results demonstrate that significant levels of plasmolysis occur during the cryopreservation procedure.