ARS | Publication request: GENE EXPRESSION PHENOTYPES ASSOCIATED WITH FLOODING TOLE RANCE OF SAG12:IPT ARABIDOPSIS

Submitted to: Mid Atlantic Plant Molecular Biology Society Conference
Publication Type: Abstract Only
Publication Acceptance Date: April 20, 2004
Publication Date: August 19, 2004
Citation: Vantoai, T.T., Liu, F., Moy, L., Huynh, L., Bock, G., Banowetz, G.M., Quackenbush, J. 2004. Gene expression phenotypes associated with flooding tole rance of sag12:ipt arabidopsis [abstract]. Mid Atlantic Plant Molecular Biology Society Conference. p. 16. .

Technical Abstract: Transgenic Arabidopsis plants (IPT) containing the SAG12:ipt autoregulated cytokinin production capability accumulate more cytokinin and remain greener and produce more biomass and seeds than wild-type (WT) plants under flooding stress. To gain insight into changes in gene expression that contribute to flooding tolerance, whole genome microarray analysis of the 27,000 Arabidopsis genes was conducted at specific times during a 24-hour period of non-flooded control, waterlogging and complete submergence. Hierarchical clustering using Euclidean distance metric revealed distinct patterns of gene expression in these treatments. Under stresses, genes of photosynthesis and energy utilization pathways expressed 2- to 4-fold more in the tolerant IPT plants than in WT plants suggesting that flooding tolerance mechanisms involve both energy production and utilization. Transcript levels of genes involved in transport (oxygen, carbohydrates, lipids, and hormones), disease resistance, stress protection and AP2/EREBP transcription factor increased significantly in IPT plants under submergence stress, but not in WT plants. These cytokinin-responsive genes probably play with a critical role in delaying plant senescence and improving flooding tolerance.