ARS | Publication request: DEVELOPMENT OF MOLECULAR MARKERS FOR INTROGRESSION OF VIRAL RESISTANCE GENES FROM SOLANUM ETUBEROSUM.

Submitted to: American Journal of Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 14, 2003
Publication Date: January 1, 2004
Citation: Gillen, A.M., Novy, R.G. 2004. Development of molecular markers for introgression of viral resistance genes from solanum etuberosum.. American Journal of Potato Research. 81(1):61

Technical Abstract: Potato Virus X (PVX), potato virus Y (PVY) and potato leafroll virus (PLRV) are important viral pathogens of potato. Solanum etuberosum, a wild relative of potato, is a source of resistance to these viruses that has yet to be fully exploited by plant breeders. A 1 EBN species, S. etuberosum cannot be readily crossed to diploid or tetraploid clones of the cultivated potato. Therefore, somatic hybridization between S. etuberosum (2n=2x=24) and a Gp. Tuberosum haploid x S. berthaultii hybrid (2n=2x=24) was employed. Somatic hybrids have been successfully crossed to cultivated potato (2n=4x=48) and resistances to PVX, PVY and PLRV have been found to segregate in the BC2 progeny. The S. etuberosum genome (E genome) is distinct from the cultivated potato genome (A genome). The impact of homology on intergenomic recombination and the possible preferential transmission of certain S. etuberosum chromosomes are being investigated using RFLP markers. We are using RFLP probes that have been previously mapping in tomato and potato (A genome and E genome) to correlate S. etuberosum chromosomal regions and disease resistance phenotypes in the BC2. RFLP markers will facilitate localizing the PVX, PVY and PLRV resistance genes in the potato genome(s). These molecular markers combined with GISH may detect desirable A and E genome recombinant genotypes, or , if no recombinants are found, progeny can be screened to select individuals that retain chromosomal segments correlated with viral resistance. Further marker saturation and analysis in the BC3 will be done to identify markers closely linked to resistance genes that can be used for marker-assisted selection.