ARS | Publication request: THE USE OF INFECTIOUS CLONES TO EXAMINE CONTRIBUTIONS OF VARIOUS GENES TO VIRULENCE OF NEWCASTLE DISEASE VIRUS

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: March 9, 2004
Publication Date: July 24, 2004
Citation: Wakamatsu, N., King, D.J., Seal, B.S., Peeters, B., Samal, S., Brown, C. 2004. The use of infectious clones to examine contributions of various genes to virulence of newcastle disease virus. American Association of Avian Pathologists.

Technical Abstract: Infectious clones of Newcastle disease virus (NDV) were utilized to evaluate relative contributions of the fusion (F) protein, hemagglutinin-neuraminidase (HN) protein, and phosphoprotein (P) to virulence. Specifically, two different clones of the La Sota virus backbone with a F cleavage site mutated to virulent motif, HN chimeras of avirulent La Sota/virulent Beaudette C, and a virulent Beaudette C backbone with mutations in P were examined. Four-week-old White Leghorn chickens were inoculated with viruses, and tissues collected at intervals were examined by histology, immunohistochemistry, and/or in situ hybridization. Birds inoculated with recombinant La Sota with virulent F or virulent (Beaudette C origin) HN genes had histologic lesions and viral distribution that were greater than the lentogenic parent yet