|Lyon, Stephen - ORANGE CTY WATER DIST.|
Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: February 20, 2004
Publication Date: April 1, 2004
Citation: Ibekwe, A.M., Lyon, S. 2004. Impact of dairy production on microbial characteristics through drinking water aquifer materials. (CD-ROM). American Society for Microbiology Annual Conference, New Orleans, LA. May, 2004. Technical Abstract: Public health concerns about pathogens present in animal manure are emerging constraints to water supplies in many areas of the world. The aim of this study was to examine microbial community composition in aquifer material in an effort to understanding key processes that control the growth and mobility of pathogens through aquifer sediment. Total microbial community profiles were compared using eubacterial primers to amplify 16S rRNA genes from total bacterial DNA and RNA. PCR and reverse transcriptase (RT) PCR were used to amplify 16S ribosomal RNA, and the products were subjected to denaturing gradient gel electrophoresis (DGGE). Prominent DGGE bands were excised and sequenced to gain insight into the identities of predominantly present (PCR) and predominantly active (RT-PCR) bacterial populations. In addition, 16S rRNA genus-specific primers sets for Enterococcus were used to obtain microbial fingerprint of the indigenous Enterococcus population present in the aquifer material. A more detailed analysis with sequencing of 16S rRNA fragments showed a wide variety of cells from source water initially attached to the aquifer materials and resulted in species richness. At the end of the experimental period, the biofilm entered a stable population state, which was characterized by a greater richness of bacteria, including Nitrospira, Planctomyces, Acidobacterium, and Pseudomonas.