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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of Microbial Communities in Cecal and Fecal Samples from Pigs Fed Two Levels of Cellulose and Protein.

item Ziemer, Cherie
item Kerr, Brian

Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: May 4, 2004
Publication Date: August 22, 2004
Citation: Ziemer, C.J., Davidson, S.K., Stahl, D.A., Kerr, B.J. 2004. Comparison of microbial communities in cecal and fecal samples from pigs fed two levels of cellulose and protein.. Meeting Proceedings. p. 267.

Technical Abstract: Information on microbial community structure of swine GIT is growing; however, studies comparing effects of dietary changes are limited. Our initial investigation was designed to evaluate the effects of protein (14.5% vs. 12% crude protein) and fiber (2.5% vs. 8.7% cellulose) levels on microbiology in swine cecum and feces. Pigs (n=10) were fed 1.5 kg twice daily, adapted to control diet for 2.5 wk then fed experimental diets for 8 wk. Cecal and fecal samples were collected weekly. Cellulose addition increased fecal output (973 vs. 686 g/d). No treatment effects were apparent for pH (6.45, 6.79) or total anaerobes (9.41, 9.33 log CFU/ml) in either cecal or fecal samples. Initial microbial community analysis used group specific ARDRA for total bacterial and 2 clostridial groups. DNA extracted from fecal and cecal samples (Week 0 and Week 5), one pig per treatment, was PCR amplified using group-targeted primers, restriction enzyme digested and visualized on agarose gels. While there were some differences among animals, banding patterns of Week 0 samples were remarkably similar for the groups assessed. Changes in banding patterns were observed with treatments for total bacteria, with a tendency for fewer bands in Week 5 samples. Few differences were observed in analysis of the group including Clostridium lentocellum, and Roseburia cecicola. For the other clostridial group (C. sticklandii, Selenomonus sp.), fecal samples had fewer bands than cecal, while cellulose increased and low protein decreased number of bands in Week 5 cecal samples. A second replication is ongoing with 12 additional pigs.

Last Modified: 4/19/2015
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