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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Size Analysis of Extracted Citrus Pectins by Multiangle Light Scattering

Author
item Luzio, Gary

Submitted to: Annual Meeting of the Institute of Food Technologists
Publication Type: Abstract Only
Publication Acceptance Date: July 9, 2004
Publication Date: July 16, 2004
Citation: Luzio, G.A. 2004. Molecular size analysis of extracted citrus pectins by multiangle light scattering. Annual Meeting of the Institute of Food Technologists. Book of Abstracts. p. 147. Paper No. 56-6.

Technical Abstract: The goal is to produce new products from citrus peel tissue that remains after juicing. After washing, citrus peel tissue is largely composed of pectin, cellulose and hemicellulose. In order to utilize the greatest amount of peel byproduct, it is reasonable that one or all three of these polysaccharides be converted to a useful product. One component, pectin, is relatively easy to modify using enzymes and has great utility in the food industry. The initial focus is on the use of pectin for the maximum utilization of citrus peel for new products. As with most water-soluble polymers, such as pectin, rheology is an important parameter and is directly related to the polymer molecular weight. Molecular weight is also affected during the procedure used to release pectin. Thus for many new products being developed from pectin one needs to be able to accurately characterize its molecular weight. One of the most effective means of determining accurate molecular weights of water soluble polymers such as pectin is through the use of multi-angle laser light scattering (MALLs) detector coupled to an interferometric refractometer. Characterization of aggregation and disaggregation states on crude extracts can be monitored using the MALLS liquid chromatography system in a static mode if molecules are too large to permit the use of size exclusion chromatography (SEC). In less aggregated states MALLS coupled to SEC is a useful tool. MALLS can also measure radius of gyration and that may be useful information for process filtration.

Last Modified: 11/26/2014
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