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United States Department of Agriculture

Agricultural Research Service

Title: High-Throughput Analysis of Barley Gene Expression in Wheat-Barley Chromosome Addition Lines

Authors
item Muehlbauer, Gary - UNIVERSITY OF MINNESOTA
item Cho, Seungho - UNIVERSITY OF MINNESOTA
item Garvin, David

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2004
Publication Date: November 1, 2004
Citation: Muehlbauer, G.J., Cho, S., Garvin, D.F. 2004. High-throughput analysis of barley gene expression in wheat-barley chromosome addition lines [abstract]. ASA-CSSA-SSSA Annual Meeting Abstracts. Paper No. 5354.

Technical Abstract: Chinese Spring wheat-Betzes barley addition lines offer an opportunity to examine many aspects of alien gene expression in plants. We used the Barley1 Affymetrix GeneChip, which represents 22,860 barley genes, to examine gene expression in Betzes, Chinese Spring, and Chinese Spring-Betzes chromosome addition lines. In young seedlings, we detected 7,811 genes in Betzes, and 6,472 of these were not detected in Chinese Spring. In the 6H addition line, 423 of the barley genes exhibited 6H-specific expression, and the use of 6HL and 6HS ditelo addition lines revealed that 239 and 184 of these genes showed 6HL- and 6HS-specific expression, respectively. Fifty genes showed a quantitative increase in expression in the 6H addition line relative to Chinese Spring. These data provide evidence that these 473 genes map to chromosome 6H. Additional analyses showed that chromosomes 3H, 4H, 5H, and 7H are highly similar in terms of gene expression profiles, chromosome 6H is moderately similar, and 2H exhibits the most unique transcript profile. Thus, microarray-based analysis of barley gene expression in these addition lines is an efficient method to physically map large numbers of genes to specific barley chromosomes, has provided unique insights into alien gene expression in wheat, and has revealed varying degrees of relatedness between the transcriptomes of different barley chromosomes.

Last Modified: 9/21/2014
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