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United States Department of Agriculture

Agricultural Research Service

Title: Effects of Temperature on Desaturase Allele Expression at Various Stages of Seed Development in Soybean

Authors
item Byfield, Grace - NCSU
item Xue, Huiqin - NCSU
item Upchurch, Robert

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 19, 2004
Publication Date: July 19, 2004
Citation: Byfield, G.E., Xue, H., Upchurch, R.G. 2004. Effects of temperature on desaturase allele expression at various stages of seed development in soybean [abstract]. Soy2004 Conference, Columbia, MO, 08-11 August 2004.

Technical Abstract: Fatty acid desaturases (FADs) play an important role in determining the fatty acid composition of soybean oil during seed development. Soybean lines have been developed that produce oil containing 50% or greater oleic acid (C18:1) This higher level of oleic acid is nutritionally desirable and beneficial for human health. The stable expression of the mid oleic trait in soybean, however, is significantly affected by plant growth temperature. In an effort to document and understand the effect of temperature on fatty acid composition of the oil, we conducted experiments to examine the potential interaction between temperature and the expression of seven desaturase alleles at various stages of seed development in five soybean cultivars. Specific primers were designed and used to survey the genomes of selected soybean lines for the presence of FAD alleles using polymerase chain reaction (PCR). Work in progress and published work have identified the presence of alleles for stearoyl acyl-carrier protein desaturase (SACPD) ' two potential alleles, FAD2-1 ' two alleles and FAD3 ' three alleles. Plants were grown under controlled temperatures and beans harvested at various growth stages between R5 and R6. Total RNA was isolated and allele-specific primers used to quantify transcript accumulation of the various alleles by reverse transcription, Real Time PCR. Results suggest that there is significant interaction between temperature and transcript accumulation in all the lines investigated. Differences were also observed in transcript accumulations for the various stages that were analyzed.

Last Modified: 9/10/2014
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