Technical Abstract: Growth chamber experiments were conducted to evaluate chemical alterations in the rhizosphere of mycorrhizal wheat roots after inoculation with Glomus intraradices (AMF). Exchange resins were used as sinks for nutrients to determine whether the inoculated plant can utilize the unavailable sources of P and micronutrients. Treatments included: i. Soil (bulk soil); ii. AMF inoculation no P addition (I-P); iii. No inoculation with no P addition (NI-P); iv. AMF inoculation with the addition of 50 mg P kg-1 soil (I+P) and v. No inoculation with the addition of 50 mg P kg-1 soil (NI+P). Spring wheat (Triticum aestivum cv. 'Len') was planted into each plexiglass pot and grown in a growth chamber for two weeks where water was maintained at field capacity. Rhizosphere pH and Eh, nutrient bioavailability indices and mycorrhizal colonization were determined. Mycorrhizal inoculation increased root colonization by the fungi when P was not added. The rhizosphere pH was lower in the inoculated pots compared to the non-inoculated while the Eh did not change. The decrease in pH in the rhizosphere of inoculated plants may have increased P and Zn uptake with inoculation. In contrast, Mn uptake was decreased by inoculation. The resin-adsorbed P was increased by inoculation which, along with the bioavailability index data, may indicate that mycorrhizal roots were able to utilize unavailable forms of soil P.