|Park, Sung Hun - TEXAS A&M UNIV|
|Morris, Jay - TEXAS A&M UNIV|
|Park, Jung Eun - TEXAS A&M UNIV|
|Smith, Roberta - TEXAS A&M UNIV|
Submitted to: Journal of Plant Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 10, 2003
Publication Date: October 10, 2003
Citation: Park, S., Morris, J.L., Park, J., Hirschi, K., Smith, R.H. 2003. Efficient and genotype-independent agrobacterium - mediated tomato transformation. Journal of Plant Physiology. 160(10):1253-1257. Interpretive Summary: In order to increase plant productivity and increase the nutritional content of foods, it is important that we develop ways to express different genes in agriculturally important crops. In this work, we have developed a method to make agriculturally important tomato varieties express an array of different genes. Through this technology, we can start to make more nutritious and robust tomato plants.
Technical Abstract: An efficient method to transform five cultivars of tomato (Lycopersicon esculentum). Micro-Tom, Red Cherry, Rubion, Piedmont, and E6203 is reported. A comparison was made of leaf, cotyledon, and hypocotyl explants on 7 different regeneration media without Agrobacterium tumefaciens cocultivation and on 11 different media with cocultivation. Although all cultivars and explants formed callus and regenerated on the initial 7 media, cocultivation with A. tumefaciens significantly reduced the callus induction and regeneration. From these experiments, a transformation methodology using either hypocotyls or cotyledons cultured for one day on BA 1 mg L-1, NAA 0.1 mg L-1 and 3 days cocultivation with the Agrobacterium on this same medium followed by a transfer to a medium with zeatin 2 mg L-1 and IAA 0.1 mg L-1 for 4-6 weeks resulted in a greater than 20% transformation frequency for all five cultivars tested. In this transformation method, no feeder layers of tobacco, petunia or tomato suspension cultures were used, and the subculture media was minimal. Stable integration and transmission of the transgene in T1 generation plants were confirmed by Southern blot analysis. Thus procedure represents a simple, efficient and general means of transforming tomato.