Submitted to: Veterinary Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 15, 2004
Publication Date: July 15, 2005
Citation: Dubey, J.P., Benson, J.E., Blakeley, K.T., Booton, G.C., Visvesvara, G.S. 2005. Disseminated acanthamoeba sp. infection in a dog. Veterinary Parasitology 128:183-187..
Interpretive Summary: Acanthamoeba species are free-living parasites that can cause mortality in humans and animals. Scientists at the Beltsville Agricultural Research Center and the CDC, Atlanta, Georgia, report a case fatal neurologic illness in a dog. The results will be of interest to biologisits, parasitologists and veterinarians.
Several species of free-living amoebe can cause encephalomyelitis in animals and humans. Disseminated acanthamoebiasis was diagnosed in pyogranulomatous lesions in brain, thyroid, pancreas, heart, lymph nodes, and kidney of a one-year-old dog. Acanthamoeba sp. was identified in canine tissues by conventional histology, by immunofluorescence, by cultivation of the parasite from the brain of the dog that had been stored at -70'c for two months, and by PCR. The sequence obtained from the PCR product from the amoeba from the dog was compared to other sequences in the Acanthamoeba ribosomal DNA database and was determined to be genotype T1, associated with other isolates of Acanthamoeba obtained from granulomatous amebic encephalitis infections in humans.