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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #165619

Title: DETECTION, PHYLOGENETIC ANALYSIS, AND GEOGRAPHIC DISTRIBUTION OF FRAGARIA CHILOENSIS LATENT VIRUS, A NEW ILAVIRUS INFECTING STRAWBERRY IN NORTH AMERICA

Author
item TZANETAKIS, I - OREGONS STATE UNIV
item Martin, Robert

Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2004
Publication Date: 7/1/2004
Citation: Tzanetakis, I.E., Martin, R.R. 2004. Detection, phylogenetic analysis, and geographic distribution of fragaria chiloensis latent virus, a new ilavirus infecting strawberry in north america. American Society for Virology Meeting.

Interpretive Summary:

Technical Abstract: Fragaria chiloensis latent virus (FClLV) was first identified in the early 1990's. The virus was only found in plant material from South America. We have cloned and sequenced dsRNA from FClLV infected Fragaria chiloensis plants from Chile. The full genome of the virus has been sequenced, and some distinct features, not found in most ilarviruses, have been identified. A sensitive reverse transcription-polymerase chain reaction (RT-PCR) test for the detection of the virus, which amplifies a 333 base pair fragment of the polymerase gene, has been developed. Applying the RT-PCR detection method, the virus was detected in North America, a region in which FClLV had not been found previously. The phylogenetic analysis, utilizing four genes of the virus, reveals that FClLV is not closely related to Lilac ring mottle and Asparangus virus-2, as originally thought based on serological tests. But FClLV is closely related to Prune dwarf, Apple mosaic, and Prunus necrotic ringspot viruses, ilarviruses with primary hosts in the family Rosaceae as is strawberry for FCLlV.