Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 26, 2004
Publication Date: July 18, 2004
Citation: Lewers, K.S., Stafne, E.T., Clark, J.R., Weber, C.R., Graham, J. 2004. Simple sequence repeat markers for raspberry and blackberry.. Meeting Abstract.
Simple Sequence Repeat (SSR) markers for raspberry and blackberry
Researchers developing new cultivars of Rubus species (raspberry and blackberry) are becoming interested in developing molecular markers for marker-assisted selection of genotypes having traits that express late in the life of the plant. Simple Sequence Repeat molecular markers (SSRs) are desired for their many advantages. Several research groups working with SSR markers have reported that SSRs developed from one species can sometimes be used with other related species. If this is true among Rubus species, such SSR markers would be useful in transferring traits from one species to another, especially beneficial since it is not uncommon for a blackberry or raspberry breeder to utilize more than one Rubus species. Six SSRs derived from R. alceifolius, an invasive European weed, and six SSRs from R. ideas red raspberry cultivar 'Glen Moy' were tested on R. ideas red raspberry selection NY322 from Cornell University, R. occidentalis 'Jewel' black raspberry, and Rubus spp. blackberry cultivar 'Arapaho', and blackberry selection APF12 from the University of Arkansas. The two raspberry genotypes are parents of an interspecific mapping population segregating for fruit color, tipping, and bearing fall fruit. The two blackberry genotypes are parents of a population segregating for primocane fruiting and thornlessness. Of the eight R. alcefolius SSRs two amplified a product from NY322, 'Jewel', and the blackberry genotypes. Of the six R. ideas SSRs 3 amplified a product from NY322, 3 amplified a product from 'Jewel', and 4 amplified a product from the blackberry genotypes. An additional 68 R. ideas SSRs were tested only on the four Rubus mapping parents. Of the entire set of 74 red raspberry SSRs, 56 (74%) amplified a product from NY322 red raspberry, 39 (53%) amplified a product from 'Jewel' black raspberry, and 24 (32%) amplified a product from blackberry. Of the 56 SSRs that amplified a product from NY322, 17 failed to amplify a product from 'Jewel' and therefore detected polymorphisms between the parents of this interspecific mapping population. About twice as many detected polymorphisms between blackberry and red raspberry, since 33 SSRs amplified a product from NY322, but neither of the blackberry genotypes. Differences in PCR product sizes from these genotypes reveal additional polymorphisms. Rubus is among the most diverse genera in the plant kingdom, so perhaps it is not surprising that only 19 of the 74 raspberry-derived SSRs amplified a product from all four of the Rubus genotypes we tested. SSRs like these will be especially useful in interspecific mapping and cultivar development.