|Franc, Gary - UNIVERSITY OF WYOMING|
|Spence, Raina - UNIVERSITY OF WYOMING|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: May 5, 2004
Publication Date: June 1, 2004
Citation: Davidson, R.M., Hanson, L.E., Franc, G.D., Spence, R., Panella, L.W. 2004. Analysis of beta-tubulin in cercospora beticola with different benzimidazole-sensitivity.. Phytopathology. 94:S24. Technical Abstract: Cercospora beticola causes Cercospora leaf spot of sugar beet and crops in the genus Beta. On sugar beet, Cercospora leaf spot is the most important foliar disease worldwide. The primary controls are host resistance and foliar fungicide treatments. Benzimidazole fungicides are used for disease control, even though benzimidazole-resistance is increasingly widespread in the pathogen population in the United States. We examined isolates of C. beticola from different years and geographic regions for sensitivity to benzimidazole fungicides and for their beta-tubulin gene sequence. Two homologous PCR primer pairs for the beta-tubulin gene developed for ascomycetes gave inconsistent results in C. beticola, but a third set amplified well. This indicates some variability in areas of the gene proposed to be highly conserved. However, isolates with benzimidazole resistance had a mutation in the beta-tubulin gene that corresponded to mutations previously determined to confer benzimidazole resistance in other ascomycetes. A PCR-based method for detection of benzimidazole resistance is being developed.