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United States Department of Agriculture

Agricultural Research Service

Title: Integrating Linkage and Radiation Hybrid Mapping Data for Bovine Chromosome 15

Authors
item Snelling, Warren
item Gautier, M - INRA-CRJ, FRANCE
item Keele, John
item Smith, Timothy
item Stone, Roger
item Harhay, Gregory
item Bennett, Gary
item Ihara, N - SHIRAKAWA INST., JAPAN
item Takasuga, A - SHIRAKAWA INST., JAPAN
item Takeda, H - SHIRAKAWA INST., JAPAN
item Sugimoto, Y - SHIRAKAWA INST., JAPAN
item Eggen, A - INRA-CRJ, FRANCE

Submitted to: Biomed Central (BMC) Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 8, 2004
Publication Date: October 8, 2004
Citation: Snelling, W.M., Gautier, M., Keele, J.W., Smith, T.P., Stone, R.T., Harhay, G.P., Bennett, G.L., Ihara, N., Takasuga, A., Takeda, H., Sugimoto, Y., Eggen, A. Integrating linkage and radiation hybrid mapping data for bovine chromosome 15. Biomed Central (BMC) Genomics. 5:77. 2004.

Interpretive Summary: Genome maps are essential to understanding genes influencing livestock production, and provide an important scaffold to assemble genomic sequence. Linkage maps are required to identify regions of the genome influencing quantitative traits, but cattle linkage maps contain few gene markers. Radiation hybrid maps of cattle contain more markers associated with genes, but the technique is not useful to identify genomic regions influencing animal performance. Linkage and radiation hybrid data are complementary when combined into a single map, taking advantage of resolution and marker characteristics of each, resulting in a denser, more reliably ordered map that facilitates identifying genes underlying quantitative trait loci. Linkage and radiation hybrid data were simultaneously analyzed to construct an integrated map of bovine chromosome 15. Where possible, markers were aligned with genomic sequence of human chromosome 11 to produce a comparative map. Automated data integration and comparative mapping procedures were developed. The integrated and comparative maps of bovine chromosome 15 provide a resource to further explore genes underlying a previously identified quantitative trait loci for meat tenderness. Extending the procedures to the entire bovine genome will provide valuable tools for further study of other important traits, and a structure to assist assembly of the bovine genome sequence.

Technical Abstract: Genome maps are essential to understanding genes influencing livestock production, and provide an important scaffold to assemble genomic sequence. Linkage maps are required to identify regions of the genome influencing quantitative traits, but cattle linkage maps contain few gene markers. Radiation hybrid maps of cattle contain more markers associated with genes, but the technique is not useful to identify genomic regions influencing animal performance. Linkage and radiation hybrid data are complementary when combined into a single map, taking advantage of resolution and marker characteristics of each, resulting in a denser, more reliably ordered map that facilitates identifying genes underlying quantitative trait loci. Linkage and radiation hybrid data were simultaneously analyzed to construct an integrated map of bovine chromosome 15. Where possible, markers were aligned with genomic sequence of human chromosome 11 to produce a comparative map. Automated data integration and comparative mapping procedures were developed. The integrated and comparative maps of bovine chromosome 15 provide a resource to further explore genes underlying a previously identified quantitative trait loci for meat tenderness. Extending the procedures to the entire bovine genome will provide valuable tools for further study of other important traits, and a structure to assist assembly of the bovine genome sequence.

Last Modified: 8/27/2014
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