|Bouldin, Jennifer - ARKANSAS STATE UNIV|
|Farris, Jerry - ARKANSAS STATE UNIV|
|Smith Jr, Sammie|
Submitted to: Society of Environmental Toxicology and Chemistry Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 18, 2004
Publication Date: May 27, 2004
Citation: Bouldin, J.L., Farris, J.L., Moore, M.T., Smith Jr, S., Cooper, C.M. 2004. Indication of diazinon movement through constructed wetlands from in situ biomarkers and laboratory biomonitoring [abstract]. Mid-South Regional Society of Environmental Toxicology and Chemistry. p. 15. Technical Abstract: Best management practices for the modification of agricultural associated nonpoint source runoff include constructed mitigation wetlands. Following a simulated runoff from a 1.3 cm rainfall event, a series of vertical wetlands was used to measure the fate and effect of the organophosphate insecticide diazinon. Water, sediment and plant samples from five sites spaced throughout the wetland, were analyzed for diazinon concentrations from 0.5 h to 26 d. Biomarkers for sublethal responses were extracted from Corbicula fluminea deployed insitu at corresponding sites. While no acute toxicity to Pimephales promelas was measured upon exposure to site water, toxicity to Ceriodaphnia dubia progressed through the wetlands after 9 h. Toxicity was measured to C. dubia exposed to aqueous samples from the site nearest the runoff introduction through 7 d and insitu organisms at these sites experienced complete mortality by the 26 d sampling. While toxicity was measured in Chironomus tentans exposed to sediment from upstream sites at 26 d only, survival was reduced in sediments sampled after 48 h through 14 d at downstream sites. In this exposure, clam acteylcholinesterase, cellulase, and growth responses were indicative biomarkers of aqueous and sediment toxicity measured in laboratory tests, while chemical analyses confirmed the movement of diazinon through the wetland cells.