Submitted to: International Plant Protection Congress
Publication Type: Abstract Only
Publication Acceptance Date: March 28, 2004
Publication Date: May 13, 2004
Citation: Huang, Y., Burd, J.D., Porter, D.R. 2004. Profiling of differentially expressed genes in sorghum as incited by greenbug attack [abstract]. In: Proceedings of the 15th International Plant Protection Congress, May 11-16, 2004, Beijing, China. p. 243. Technical Abstract: Host-plant resistance is a major component in the development of control strategies against insect pests that attack agricultural crops. Thus many techniques have been developed for screening germplasm and identified sources of resistance have already been used in the breeding program. Conventional breeding programs to enhance the levels of resistance to certain insect pests have been successful through large efforts of many breeders over a long period of time. Recently, successful development in functional genomics using DNA chip (microarray) technology and large-scale DNA sequencing offers powerful tools for rapid identification of genes responsible for host resistance to insects that cause significant damage to crop production. In this study, we have employed the emerging technology combining suppression subtractive hybridization (SSH) and cDNA microarrays for a high throughput analysis of gene expression in sorghum (Sorghum bicolor L. Moench) in response to attack by greenbug (Schizaphis graminum), a major insect pests of many cereal crops. These cutting-edge technologies ware able to both reveal the information on the expression patterns of thousands of genes in parallel samples and allowed identification of a large number of differentially expressed genes in the sorghum plants. Determination of the function of those identified sorghum genes in combating insect attack is underway.