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United States Department of Agriculture

Agricultural Research Service

Title: Production of Est from Cdna Libraries Derived from Immunologically Activated Bovine Small Intestine

Authors
item SONSTEGARD, TAD
item VAN TASSELL, CURTIS
item Matukumalli, Lakshmi - GEORGE MASON UNIVERSITY
item HARHAY, GREGORY
item Bosak, S - AGENCOURT BIOSCIENCES
item Rubenfield, M - AGENCOURT BIOSCIENCES
item Gasbarre, Louis

Submitted to: Genbank
Publication Type: Germplasm Release
Publication Acceptance Date: May 18, 2004
Publication Date: May 25, 2004
Citation: Sonstegard, T.S., Van Tassell, C.P., Matukumalli, L.K., Harhay, G.P., Bosak, S., Rubenfield, M., Gasbarre, L.C. 2004. Production of est from cdna libraries derived from immunologically activated bovine small intestine. Germplasm Release.

Interpretive Summary: This is a Germplasm Release, no Interpretive Summary required.

Technical Abstract: Expressed sequence tag (EST) information derived from small intestine undergoing active parasite infection is critical to functional genomic studies aimed at understanding the bovine mucosal immune system and gut tissue responses (repair, necrosis, etc.). An arrayed, normalized cDNA library (BARC 10BOV) was constructed using a pooled sample of mRNA derived from various tissues induced for the stereotypic Th1 and Th2 immune responses by infection with both site-specific nematode parasites and a protozoan parasite. A total of 21,205 EST were generated from the 10BOV library. These EST serve as a resource for annotating the bovine genome, design of hybridization oligonucleotides for microarray resources, and cDNA clone sources for generating full-length reference sequences in cattle. GenBank Accession Numbers: CK940478, CK961490, CK982806, CK982904, CK983155, CK983247

Last Modified: 9/29/2014