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United States Department of Agriculture

Agricultural Research Service

Title: Cloning of Aflp Fragments Linked to Resistance Genes for Fusarium Head Blight in Barley.

Authors
item Agrama, Hesham - PLNT PATH NDSU, FARGO, ND
item Dahleen, Lynn
item Mcarthur, Rachel - PLNT SCI, NDSU, FARGO, ND

Submitted to: Proceedings International Barley Genetics Symposium
Publication Type: Proceedings
Publication Acceptance Date: May 11, 2004
Publication Date: June 20, 2004
Citation: Agrama, H.A., Dahleen, L.S., Mcarthur, R.I. 2004. Cloning of AFLP fragments linked to resistance genes for Fusarium Head Blight in barley. Proceedings International Barley Genetics Symposium. June 20-26, 2004, Brno, Czech Republic. CDROM.

Interpretive Summary: Fusarium head blight (FHB) caused by Fusarium graminearum reduces yield and quality in barley due to the toxin deoxynivalenol (DON), throughout the United States and worldwide. The development and use of cultivars with resistance to FHB is the most economically viable option for the control of this disease. We have used DNA molecular markers to identify and locate the FHB resistance genes in barley by analyzing progeny segregating for resistance and susceptibility. A three-way cross (Zhedar 2/ND9712//Foster) was made to develop the progeny population used for this study. Many DNA molecular markers were associated with resistance. Many of these markers were located on chromosomes 2H and 5H at regions closely associated with FHB resistance and low DON concentration. New markers have been developed that are easier to use than the original markers. The new markers are currently being tested to see how useful and efficient they are in identifying genes for resistance to Fusarium graminearum on chromosome 2H.

Technical Abstract: Genetic mapping of resistance genes to Fusarium head blight (FHB) in barley, caused by Fusarium graminearum, revealed multiple locus inheritance. A segregating population of seventy-five double haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster, was used for genome mapping and FHB evaluation. Four amplified fragment length polymorphism (AFLP) markers were identified and mapped to chromosomes 2H and 5H at regions closely associated with FHB resistance and low deoxynivalenol (DON) concentration. An additional AFLP marker was associated with disease susceptibility. These five AFLP fragments were purified, cloned and sequenced. Sequence-characterized amplified regions (SCARs) will be produced and used for assessing the presence of FHB resistance genes for comparisons in other barley genotypes. These markers may therefore be useful in marker-assisted selection of resistance genes in barley breeding programs and will facilitate map-based cloning.

Last Modified: 11/28/2014
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