Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only
Publication Acceptance Date: May 11, 2004
Publication Date: June 20, 2004
Citation: Grisham, M.P. 2004. Evaluation of Ho 95-988, HoCP 96-540, and L 97-128 for Susceptibility to Ratoon Stunting Disease [abstract]. Journal of the American Society of Sugar Cane Technologists. 24:110. Technical Abstract: The susceptibility of recently released sugarcane cultivars for Louisiana, HoCP 96-540 released in 2003 and Ho 95-988 and L 97-128 released in 2004, to ratoon stunting disease (RSD) was evaluated between 1999 and 2003. Single-bud cuttings were collected from each cultivar in the fall, dip inoculated in juice from stalks infected with Leifsonia xyli subsp. xyli, the bacterium that causes RSD, germinated in the greenhouse, then transplanted in the spring to a field nursery at the USDA, ARS research farm. Four mature stalks were collected from each cultivar in the plant-cane, first-ratoon, and second-ratoon crops of at least two complete crop cycles and susceptibility to infection was evaluated using tissue-blot immunoassay (TBIA). The average percent colonized vascular bundles (CVB) as determined by TBIA for Ho 95-988, HoCP 96-540, and L 97-128 was compared to the percent CVB of other cultivars currently or recently recommended for planting in Louisiana. The lowest percent CVB (2%) was found among samples of Ho 95-988. No infection was recorded for many of the stalk samples. Relatively low percent CVB was also found for L 97-128 (14%) and HoCP 96-540 (9%). The highest percent CVB was observed in HoCP 91-555 (49%). Other cultivars with high percent CVB readings were CP 70-321 (41%), CP 72-370 (27%), and LCP 82-89 (48%), and those with intermediate levels were HoCP 85-845 (24%) and LCP 85-384 (23%). Because of the lower level of infection among the three most recently released cultivars, control of RSD among these cultivars should be more effective.