|Sardanelli, Sandra - PNL, UNIV. OF MARYLAND|
Submitted to: Society of Nematology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 14, 2004
Publication Date: N/A
Technical Abstract: We are interested in the control of egg hatch by factors within cyst nematodes that either function autonomously or in response to external agents. Baseline in vitro hatching kinetics of H. glycines laboratory eggs were established using large (>10,000 eggs/assay) and small (500-1000 eggs/assay) scale systems. No differences in total percent hatch or in hatch rates were observed between the two systems, although there was some positive correlation between egg density and hatch rate. Hatching kinetics were then used to compare experimental treatments, and to compare hatch characteristics of laboratory eggs with those obtained from field samples. Hatching was retarded by mild rinsing of eggs in tap water. Two days after collection and rinsing, hatch was reduced 90% in rinsed egg preparations compared with non-rinsed controls. Treated eggs partially recovered by 7 days, with hatch rates at 60% of controls. Hatching was completely restored in laboratory eggs following application of rinse solution, whereas field eggs were much less responsive. The factor(s) involved show some heat stability. These and other potential sources of hatch regulators within the nematode are discussed.