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United States Department of Agriculture

Agricultural Research Service

Title: Lethality of Experimental Infection by Immersion of Edwardsiella Tarda in Channel Catfish (Ictalurus Punctatus)

item Wiedenmayer, Alyssa
item Klesius, Phillip
item Evans, Joyce

Submitted to: Southern Conference of Researchers in Aquatic Diseases
Publication Type: Abstract Only
Publication Acceptance Date: January 9, 2004
Publication Date: February 8, 2004
Citation: Wiedenmayer, A.A., Klesius, P.H., Evans, J.J. Lethality of experimental infection by immersion of edwardsiella tarda in channel catfish (ictalurus punctatus). 6th Annual Meeting of the Southern Conference of Researchers in Aquatic Diseases. 9th Biennial Meeting of the Fish Diagnostician's Workshop. 55th Annual Meeting of the Animal Disease Research Workers in Southern States. 19th Annual Meeting of the Southern Conference on Animal Parasites. February 8-10, 2004. Biloxi, Mississippi. 2004.

Technical Abstract: Edwardsiella tarda is the causative agent of edwardsiellosis in warmwater fish. Cultured channel catfish (Ictalurus punctatus) are commonly susceptible to infection. The objective of the present study was to determine the lethality of a wild E. tarda strain to healthy channel catfish infected by bath immersion. The channel catfish (N=180) were separated into replicate groups of 20 and exposed to varying amounts (9.28x105cfu/mL, 1.89x107cfu/mL, 6.22x107cfu/mL and 2.97x108cfu/mL) of E. tarda in 3 L bath solutions for 30 minutes. The fish were held, 20 individuals per tank, and observed for 96 hours post infection for disease signs and mortality. The results indicated lethal infection of channel catfish can be obtained via bath immersion at 6.22 x107cfu/mL and 2.97x108cfu/mL, Cumulative mortality was observed at be 78% at 6.22x107cfu/mL and 70% at 2.97x108cfu/mL. These results suggest that healthy channel catfish are susceptible to E. tarda infection by immersion without prior mechanical skin injury.

Last Modified: 4/22/2015