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United States Department of Agriculture

Agricultural Research Service

Title: Adult Immersion Tests of Acaricide Susceptibility in American and Australian Strains of Boophilus Microplus

Authors
item Jonsson, N - UNIV QUEENSLAND AUSTRALIA
item Miller, Robert
item Finn, A - UNIV QUEENSLAND AUSTRALIA
item Verrall, R - UNIV QUEENSLAND AUSTRALIA
item George, John

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: August 25, 2003
Publication Date: October 1, 2003
Citation: JONSSON, N.N., MILLER, R., FINN, A., VERRALL, R.G., GEORGE, J.E. ADULT IMMERSION TESTS OF ACARICIDE SUSCEPTIBILITY IN AMERICAN AND AUSTRALIAN STRAINS OF BOOPHILUS MICROPLUS. PROCEEDINGS of V INTERNATIONAL SEMINAR OF ANIMAL PARASITOLOGY, 1-3 OCTOBER 2003, MERIDA, YUCATAN, MEXICO. 2003. P. 86-91.

Interpretive Summary: Adult immersion tests are used to measure acaricide susceptibility in ticks. Generally, they are used in discriminating dose testing or as a full bioassay. Recently, there have been some reports that show that this test can be completed in 7 days rather than the traditional 6 weeks. We designed a series of experiments to determine if the 7 day test was a good indicator of resistance. The results show that this quicker test was not reliable for the detection of resistance. The results were highly variable. The traditional 6 week test is a better indicator of resistance for ticks.

Technical Abstract: Adult immersion tests (AITs) for acaricide susceptibility were conducted in a similar manner in Texas, USA, using the Muñoz strain and in Queensland, Australia, using the N-strain of B microplus. Data were collected on oviposition 7 d after exposure to acaricide and subjected to probit analysis. Most of the data provided a poor fit with the probit analysis model used and there were substantial differences in LC50 and LC99 between the two strains as tested in the respective laboratories.

Last Modified: 7/23/2014
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