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United States Department of Agriculture

Agricultural Research Service

Title: Identification of Differentially Expressed Genes from a Resistant Soybean-Rust Interaction Using Suppressive Subtractive Hybridization

item Choi, Jane
item Frederick, Reid

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2004
Publication Date: July 31, 2004
Citation: Phytopathology 94:S19

Technical Abstract: Soybean rust caused by Phakopsora pachyrhizi has spread to many parts of the world, most recently to Africa and South America. Although the disease is not in the continental United States, there are concerns that significant yield losses would occur if the pathogen were introduced and became established. Four single genes have been described for rust resistance, Rpp1-4, but no commercially available U.S. soybean cultivars are resistant to all isolates of rust. To understand the molecular mechanisms of rust resistance, a suppressive subtractive hybridization (SSH) was employed. A SSH cDNA library was constructed using mRNA from soybean line PI200492 (Rpp1) inoculated with P. pachyrhizi isolate HW94-1 (resistant interaction). Sequence was evaluated from 1011 cDNA clones. Using the BLAST sequence comparison algorithms, cDNA clones were classified into functional categories based on the Munich Information Center for Protein Sequences (MIPS), with unclassified/unknown function as the largest, followed by: metabolism; cell rescue, defense, cell death and aging; energy; and communication and signaling.

Last Modified: 4/17/2015
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