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United States Department of Agriculture

Agricultural Research Service

Title: Regeneration of Ten Trifolium Species Endemic to the Central African Continent

Author
item Morris, John

Submitted to: Plant Genetic Resources Newsletter
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 13, 2005
Publication Date: May 1, 2006
Citation: Morris, J.B. 2005, Regeneration of ten trifolium species endemic to the central african continent. Plant Genetic Resources Newsletter, Issue No. 144, p. 30-33.

Interpretive Summary: Clover species endemic to the African continent are limited in use because adequate seed production has not occurred. More than 400 accessions of these species are stored at the Plant Genetic Resources Conservation Unit at Griffin, GA. These species are unable to overwinter in the field at Griffin, GA, nor survive the high temperatures during the summer. A technique is reported to generate these clover species by growing the seeds in the greenhouse from mid April to the end of November. Seed regeneration produced 19 to 28,648 seed per accession of the entries when using this technique compared to 0 seeds recovered when plants were grown in the field during the winter or summer season. Thus, this technique proved extremely useful in the regeneration of plants and seed during the spring, summer, fall season.

Technical Abstract: Clover accessions, including T. baccarinii, T. bilineatum, T. calocephalum, T. multinerve, T. pichisermollii, T. quartinianum, T. ruppellianum, T. schimperi, T. steudneri, and T. tembense originating from the African continent were direct seeded into soil inside a greenhouse during mid April at a temperature range of 21/26 degrees C. After 5 to 6 months, 28 accessions of these clover species were evaluated for regeneration. High quality plants regenerated from all accessions produced 19 to 28,648 seed weighing 0.01 g to 30.4 g. The spring, summer, fall greenhouse regeneration technique can produce high quality seed from these clover species.

Last Modified: 9/23/2014
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