ARS | Publication request: TRANSFER OF MEDICAGO EST-SSRS TO PEANUT FOR GERMPLASM EVALUATION AND CROSS-SPECIES CLONING

Submitted to: American Peanut Research and Education Society Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: March 5, 2004
Publication Date: July 14, 2004
Citation: Wang, M.L., Barkley, N.L., Dean, R., Holbrook, C., Pittman, R.N. 2004. Transfer of medicago est-ssrs to peanut for germplasm evaluation and cross-species cloning. American Peanut Research and Education Society Proceedings. 36th Annual American Peanut Research & Education Society Proceedings, 7/2004, p-6.

Technical Abstract: Transfer of SSR markers is a very efficient approach for DNA marker development, especially in crops for which genomes have been not-well charazterized. In comparison to genomic SSRs, EST-derived SSRs are relatively easy to transfer from one species to another. We have selected a set of 100 EST-SSRs for across genera transfer from Medicago tuncatula to peanut. In a pilot experiment, about 20% of tested primers produced single, double or multiple amplicons in both cultivars and wild species. These amplicons will be further investigated as follows: (1) the polymorphic amplicons will be directly used for germplasm evaluation and comparative genetic mapping; (2) the non-polymorphic amplicons will be used for SNP discovery in cultivated peanuts for molecular breeding; (3) orthologs important for agricultural application will be cloned in peanut by primer-walking and 5' race PCR. Since the model plant Medicago truncatula is in the process of whole genome sequencing and many sequences will be available, exploiting its sequence information can probably provide some clues for efficient characterization of the peanut genome.