Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Salmonella Vaccination Programs in Broiler Breeders. I. Humoral and Mucosal Immune Response

Authors
item Rolon, A - UGA
item Bailey, Joseph
item Holt, Peter
item Holface, C - UGA
item Wilson, J - UGA
item Cosby, Douglas
item Richardson, Larry
item Cox, Nelson

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 4, 2004
Publication Date: July 27, 2004
Citation: Rolon, A., Bailey, J.S., Holt, P.S., Holface, C.C., Wilson, J.L., Cosby, D.E., Richardson, L.J., Cox, Jr., N.A. 2004. Salmonella vaccination programs in broiler breeders. I. H0umoral and mucosal immune response. Poultry Science Association Meeting Abstract. Poultry Science. 83(S1):146.

Technical Abstract: Although vaccination against Salmonella has been used more frequently in broiler breeders in recent years, there is a paucity of information in the literature demonstrating the immunological response of treatments that combine live and autogenous killed cell vaccines. The present research was designed to assess the immunological response that was generated by three vaccination protocols. Treatment vaccines consisted of a live Aro-A mutant commercial Salmonella Typhimurium (ST) vaccine and an autogenous killed vaccine consisting of a pool of Salmonella serovars Berta, Heidelberg, and Kentucky prepared by a commercial company. Four groups of 250 Cobb x Cobb breeder chicks were vaccinated as follows: A) 2 live (day 1 and 21) and 2 killed (day 77 and 126); B) 3 live (day 1, 21, and 77) and 1 killed (day 126) and ; C) 2 killed (day 77 and 126); and D) untreated controls. To assess humoral and mucosal immune response, samples of serum (SER), crop lavage (CL), gut lavage (GL), hatchling serum (HSER), and egg yolk were tested to measure IgA and IgG. ELISA for IgA and IgG on Salmonella Enteritidis and Salmonella Typhimurium lypopolysaccharide (SELPS and STLPS) as capture antigen were conducted. Overall, immunological response was stronger on STLPS than SELPS. IgA of SER, CL and GL as well as IgG of CL were short-lived peaks after first killed vaccine. Strong GL IgG after first live and both killed vaccine events were measured with the killed response enduring longer. SER IgG responses were observed after killed vaccine events, and lasting throughout 40 wk of age. HSER and EY IgA were negligible, and IgG comparable among all treatments throughout time. These results show that killed antigen is vital in eliciting adequate IgG inserum and gut. Live vaccination with Aro-A mutnat ST vaccine enhances gut IgG and possibly aids in conferring adequate immunity during the breeder's first weeks of life.

Last Modified: 10/22/2014
Footer Content Back to Top of Page