Submitted to: International Aquaculture Meeting
Publication Type: Abstract Only
Publication Acceptance Date: January 16, 2004
Publication Date: March 1, 2004
Citation: McNulty, S.T., Evans, J.J., Klesius, P.H. 2004. Development of a method to differentiate channel catfish ictalurus punctatus fillets from impoted basa pangasius sp. fillets based on methylated fatty acid (fame) profiles. Aquaculture 2004. Aquaculture 2004 March 1-5, 2004, Honolulu, Hawaii. p. 392 Book of Abstracts. Technical Abstract: A method for the differentiation of channel catfish (Ictalurus punctatus) from Vietnamese basa (Pangasius sp.) by fatty acid analysis is described. The method used the Sherlock software produced by MIDI (Newark, DE, USA) and a GC 6850 gas chromatograph (Agilent Technologies) to create a library composed of fillet methylated fatty acid (FAME) profiles. Both channel catfish and basa fillet libraries were developed. Twenty-five mg of fillet meat was cut from frozen fillets and processed according to the extraction procedure provided by MIDI. The channel catfish and basa fillet libraries were validated by the Sherlock Validation Tool. In a single blind study the FAME libraries correctly identified 94% of the fillet samples. Of the 50 fillet samples, 5 samples had to be diluted and run again, all of which were then correctly identified. Only two channel catfish fillet samples in the blind study were misidentified as basa fillets. This FAME method offers a rapid and accurate technology to identify channel catfish fillets.