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Submitted to: Meeting Abstract
Publication Type: Other Publication Acceptance Date: 3/9/2004 Publication Date: 5/22/2004 Citation: Niedz, R.P. 2004. Regeneration of somatic embryos from sweet orange (c.sinensis (l.) osbeck) protoplasts using semi-permeable membranes. Meeting Abstract. Date of Meeting May 22, 2004. Interpretive Summary: Technical Abstract: Sweet orange (C. sinensis L. Osbeck) protoplasts were isolated from nucellar-derived embryogenic callus, cultured in alginate beads for 5-30 days, and the resulting p-calli released by liquefaction and cultured on semi-permeable membranes overlaid on MT culture medium. Somatic embryos did not develop from 5 or 10 day old p-calli but did develop from 15, 20, 25, and 30 day old p-calli. There were no significant differences in the numbers of embryos produced among the 15-30 day old p-calli and no abnormal embryo morphologies were observed. The minimum size of p-calli to form embryos was 77.84 um in diameter. Embryos were substantially smaller from p-calli than those produced from embryogenic callus and averaged 200 um and >1 mm in diameter, respectively. Embryos produced from p-calli of all ages did not germinate. |
