ARS | Publication request: RECIPROCAL MONOPHYLY OF POTYVIRUS AND RYMOVIRUS GENERA, FAMILY POTYVIRIDAE, IS SUPPORED BY GENOME SEQUENCES OF AGROPYRON MOSAIC VIRUS AND HORDEUM MOSAIC VIRUS

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2004
Publication Date: July 15, 2004
Citation: French, R.C., Stenger, D.C. 2004. Reciprocal monophyly of potyvirus and rymovirus genera, family potyviridae, is suppored by genome sequences of agropyron mosaic virus and hordeum mosaic virus. American Phytopathological Society Abstracts. Phytopathology 94: S32.

Technical Abstract: Assignment of mite-transmitted virus species to the genus Rymovirus, family Potyviridae has changed several times, and the status of the genus has been questioned. To address this issue,complete nucleotide sequences of Agropyron mosaic virus(AgMV)and Hordeum mosaic virus (HoMV) were determined. AgMV(9540 nt)and HoMV (9463 nt) each encode a single polyprotein with predicted cleavage products typical of potyviruses, and share 68.5% (nt) and 71.6% (aa) sequence identity. Both are equidistant (53% nt, 49% aa sequence identity) from a third rymovirus, Ryegrass mosaic virus (RGMV). Phylogenetic analyses by neighbor joining, maximum parsimony, and Bayesian inference each group the three Rymovirus species in an exclusive clade with a most recent common ancestor basal to a clade containing 34 species of the genus Potyvirus. Because AgMV, HoMV, and RGMV share a reciprocal monophyletic relationship with species of the genus Potyvirus, and are divergentin sequence and type of vector, the genus Rymovirus remains to be a usefu taxonomic unit within the family Potyviridae.