Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: A rapid spectrophotometric method to determine beta-carotene content in cucumis melo

Authors
item Davis, Angela
item Fish, Wayne
item Collins, Julie
item Perkins Veazie, Penelope

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: February 27, 2004
Publication Date: June 1, 2004
Citation: Davis, A.R., Fish, W.W., Collins, J.K., Perkins Veazie, P.M. 2004. A rapid spectrophotometric method to determine beta-carotene content in cucumis melo. HortScience. 39(3):654.

Technical Abstract: The compound B-carotene is the predominant carotenoid in cantaloupe. Because of its antioxidant properties and health benefits, the B-carotene content in fruits and vegetables is of interest to the food industry. Current methods to assay B-carotene content in fruit are time consuming, expensive and use hazardous organic solvents. Here, we report preliminary data on a method to detect B-carotene content on 30 cantaloupe purees using light absorbance measured with a xenon flash colorimeter/spectrophotometer. Our puree absorbance method had a linear correlation coefficient with B-carotene content by hexane extraction/spectrophotometry of R2=0.7. This linear correlation shows that this method may work for quantitating B-carotene content in purees of fresh cantaloupe. Since pureeing the sample is the only processing required and no chemicals are needed, the method is rapid, inexpensive and is environmentally friendly.

Last Modified: 11/26/2014