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United States Department of Agriculture

Agricultural Research Service

Title: Detection of Moniliformin in Maize Using Capillary Zone Electrophoresis

Author
item Maragos, Chris

Submitted to: Journal of Food Additives & Contaminants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 4, 2004
Publication Date: August 1, 2004
Citation: Maragos, C.M. 2004. Detection of moniliformin in maize using capillary zone electrophoresis. Journal of Food Additives & Contaminants. 21(8):803-810.

Interpretive Summary: The fungi Fusarium proliferatum and F. subglutinans are common pathogens of maize. These fungi produce several mycotoxins including moniliformin, which is toxic to both plants and animals. Testing for moniliformin is currently done predominantly with high performance liquid chromatography. This report describes the development of a more rapid method for detection of moniliformin in maize, that uses capillary zone electrophoresis. The shorter analysis time is beneficial when testing maize for this mycotoxin.

Technical Abstract: Moniliformin (MON) is a mycotoxin produced by certain fungi that are pathogenic to maize. MON is capable of causing disease in domestic animals, possibly through inhibition of pyruvate dehydrogenase. Testing for MON commonly involves extraction of maize, isolation of MON using solid phase extraction (SPE) columns and detection with high performance liquid chromatography (HPLC), or gas chromatography. Reported herein is a capillary zone electrophoresis-diode array detection (CZE-DAD) method for determination of MON in maize. The extraction and isolation procedures are similar to those of a commonly used HPLC method, while the detection step requires only 10 min. Sixty-three samples of maize were tested by an established HPLC method using absorbance at 229 nm (HPLC-UV) and by the CZE-DAD method. The limit of detection of the CZE-DAD method was 0.1 ug MON/g maize, compared to 0.05 ug/g for the HPLC-UV method. The CZE-DAD method gave good agreement with the HPLC-UV method for samples tested at levels up to 1500 ug/g, with a linear regression having r2=0.996.

Last Modified: 12/20/2014
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