Technical Abstract: Moniliformin (MON) is a mycotoxin produced by certain fungi that are pathogenic to maize. MON is capable of causing disease in domestic animals, possibly through inhibition of pyruvate dehydrogenase. Testing for MON commonly involves extraction of maize, isolation of MON using solid phase extraction (SPE) columns and detection with high performance liquid chromatography (HPLC), or gas chromatography. Reported herein is a capillary zone electrophoresis-diode array detection (CZE-DAD) method for determination of MON in maize. The extraction and isolation procedures are similar to those of a commonly used HPLC method, while the detection step requires only 10 min. Sixty-three samples of maize were tested by an established HPLC method using absorbance at 229 nm (HPLC-UV) and by the CZE-DAD method. The limit of detection of the CZE-DAD method was 0.1 ug MON/g maize, compared to 0.05 ug/g for the HPLC-UV method. The CZE-DAD method gave good agreement with the HPLC-UV method for samples tested at levels up to 1500 ug/g, with a linear regression having r2=0.996.