Submitted to: Pig Veterinary Society International Congress Proceedings
Publication Type: Proceedings
Publication Acceptance Date: April 20, 2004
Publication Date: July 1, 2004
Citation: Register, K.B., Sacco, R.E., Brockmeier, S. 2004. Immune response to a dna vaccine derived from the pasteurella multocida toxin gene. Pig Veterinary Society International Congress Proceedings. p. 200.
Pasteurella multocida is a widespread and costly pathogen responsible for both atrophic rhinitis and pneumonia in swine. The P. multocida toxin (PMT) is a primary virulence factor in atrophic rhinitis and a major protective immunogen. Technical difficulties and expense prevent the purification of PMT in amounts suitable for widespread use as a stand-alone vaccine or as an additive to existing vaccines. DNA vaccine technology may overcome these limitations and provide a practical solution for improving vaccine efficacy. In the present study, a PMT gene fragment truncated at the 5'end but predicted to encode the major immunogenic epitopes was cloned into 2 eukaryotic expression vectors and used to vaccinate mice. Humoral and cellular immune responses were analyzed to determine the suitability of the constructs for use as DNA vaccines.