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United States Department of Agriculture

Agricultural Research Service

Title: Status Report on Introgression of Reniform Nematode Resistance from Gossypium Longicalyx

Authors
item Robinson, Arin
item Bell, Alois
item Dinghe, Nilesh - TEXAS A&M UNIVERSITY
item Stelly, David - TEXAS A&M UNIVERSITY

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Proceedings
Publication Acceptance Date: January 8, 2004
Publication Date: February 21, 2004
Citation: Robinson, A.F., Bell, A.A., Dinghe, N., Stelly, D. 2004. Status report on introgression of reniform nematode resistance from Gossypium longicalyx. In: Proceedings of the Beltwide Cotton Conferences, January 5-9, 2004, San Antonio, Texas. 2004 CDROM.

Technical Abstract: The reniform nematode (Rotylenchulus reniformis) is an increasing problem in the central cotton belt of the United States. No cultivars or breeding lines of Upland cotton (Gossypium hirsutum) have sufficient resistance to the nematode to suppress populations appreciably in soil. Of six Gossypium species where resistant accessions are reported, only Gossypium longicalyx is immune and prevents all nematode reproduction. Although G. longicalyx is an F-genome diploid that will not cross with G. hirsutum, a low incidence (ca. 1%) of seed set can be obtained by crossing G. hirsutum onto two male-sterile triple-species hybrids developed by A.A. Bell of the USDA. The hybrid denoted HLA has G. armourianum as the third species in its background while the hybrid denoted HHL has G. herbaceum. On both hybrids, nematode reproduction was undetectable in pots, although low rates (<1%) of maturation of nematodes on roots were apparent in exhaustive root examinations. Both hybrids show morphological traits of G. longicalyx as well as their other parents and are presumed to carry a complete F-genome set of chromosomes inherited from G. longicalyx, in combination with either a D genome set from G. armourianum or an A genome set from G. herbaceum. The objective of this research was to evaluate the feasibility of introgressing reniform nematode resistance from these hybrids into G. hirsutum, in order to provide breeding lines that can be utilized to develop reniform nematode-resistant cultivars of Upland cotton. We repeatedly backcrossed the hybrids with G. hirsutum and selected for nematode resistance at each generation, to obtain more than 1,100 progeny from the hybrids; 96 immune plants with increasing levels of fertility were recovered altogether in first, second and third backcross (BC1, BC2, BC3) generations. Within specific crosses, approximately 25% of backcross progeny were immune, consistent with a single (or linked) dominant gene(s). Transfer of immunity via pollen to the BC2 and BC3 was demonstrated and seed obtained by pollinating emasculated Delta and Pineland 458 and Acala NemX flowers with pollen from immune BC3 plants are ready for testing. Approximately 4,200 cells in 140 plants were cytogenetically analyzed. Eighty BC2 plants showed chromosome counts of 53-54 in 6 plants, 49-51 in 10 plants, and 52 in 64 plants; thus chromosome numbers typically were at or approaching the normal 52 for G. hirsutum. At least 2 of 10 resistant plants analyzed showed evidence of recombination at 10% or more the normal rate. In conclusion, our data on both nematode resistance inheritance and cotton cytogenetics support the feasibility of utilizing existing progeny from the HLA and HHL hybrids to transfer reniform nematode resistance into agronomic types of Upland cotton via conventional plant breeding protocols.

Last Modified: 12/25/2014