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United States Department of Agriculture

Agricultural Research Service

Title: Putrescine Improves Somatic Embryogenesis and Plant Regeneration in Select Cotton (Gossypium Hirsutum L.) Genotypes

item Sakhanokho, Hamidou
item Ozias-Akins, Peggy - UNIV OF GEORGIA
item Lewis, Kippy - UNIV OF GEORGIA
item Vickers, Jennifer - UNIV OF GEORGIA
item Chee, Peng - UNIV OF GEORGIA

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 24, 2003
Publication Date: January 21, 2003
Citation: Sakhanokho, H.F., Ozias-Akins, P., May, O.L., Lewis, K., Vickers, J., Chee, P.W. 2003. Putrescine improves somatic embryogenesis and plant regeneration in select cotton (gossypium hirsutum l.) genotypes. ASA-CSSA-SSSA Annual Meeting Abstracts.

Technical Abstract: An efficient plant transformation system depends, in large part, on the capability of the cells to produce somatic embryos (SEs) and to then produce plants. Improvement in somatic embryogenesis has been acheived in several Georgia and Pee Dee cotton lines with media containing various putrescine (Put) concentrations. The best results were obtained with the a-Naphthalene acetic acid (NAA)-based treatments, S15g.05NAA and EMMS(2), as compared to the 2,4-Dichlorophenoxyacetic acid (2,4-D)-based medium, EMMS(4). Inclusion of 0.5 mg 1(-1) Put improved SE production for the most treatments and genotypes tested. An 8- and 2-fold improvement was acheived in SE production on the EMMS(2)-0.5 Put treatment as compared to EMMS(2) alone for cotton lines PD 97019 and GA 98033, respectively. A dramatic increase in SE number (53-fold) was obtained with the addition of 0.5 mg 1(-1) Put to EMMS(2) for the genotype PD 97021, which was essentially recalcitrant without Put treatment. Conversion of SEs into plants was both genotype- and medium dependant. These results could pave the way for the direct transformation of these elite Georgia and Pee Dee cotton lines for transgenic cultivar development.

Last Modified: 4/22/2015
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