|Kamenova, Ivanka - USDA, ARS, USHRL|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 1, 2004
Publication Date: March 1, 2004
Citation: Kamenova, I., Adkins, S. T. 2004. Identification of Tomato Mosaic Virus and Tomato Ringspot Virus in Jasmine in Florida. Meeting Abstract. Technical Abstract: Virus-like symptoms were recently observed on leaves of landscape and nursery wax (Jasminum gracile) and downy and star (J. multiflorum) jasmine in southeast Florida. Foliar symptoms included chlorotic ringspots and chlorotic line patterns. An agent was mechanically transmitted with difficulty from symptomatic leaves of downy jasmine to Nicotiana debneyi and subsequently from this host to Chenopodium quinoa and other herbaceous test plants. Virions were isolated from N. tabacum cv. Xanthi. Rod-shaped particles similar to tobamoviruses and measuring ~300 x 18 nm were observed in partially purified virus preparations, and in leaf dips prepared from symptomatic jasmine leaves and indicator plants. Extraction of viral-associated double-stranded RNA revealed a profile consistent with that of a tobamovirus. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that virions contained one polypeptide with Mr of ~18 kDa. Virions reacted positively in double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using commercially available (Agdia Inc., Elkhart, IN, USA) antiserum for Tobacco mosaic virus (TMV) and related tobamoviruses. Reverse transcription-polymerase chain reaction (RT-PCR) was performed with total RNA isolated from symptomatic leaves of downy and star plants and from N. tabacum cv. Xanthi with ToMV coat protein specific primers. The expected size amplification product of 480 base pairs was obtained from samples tested, and the nucleotide and amino acid sequence of the product showed 100% identity with the corresponding fragment of a Brazilian isolate of ToMV (GenBank Accession No. AY063743 and AAL38654.1, respectively). Additionally, serological evidence was obtained for Tomato ringspot virus (ToRSV) infection of downy, star and wax jasmine tissues (leaves, bark and flowers) by DAS-ELISA and of leaves by tissue blot immunoassay (TBIA). A limited survey (DAS-ELISA and TBIA) revealed that both ToMV and ToRSV are widespread in jasmine in the Florida landscape. To the best of our knowledge, this is the first report of isolation of ToMV from jasmine and the first identification of ToMV and ToRSV infection in jasmine in the USA.