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United States Department of Agriculture

Agricultural Research Service

Title: Shuffling DNA Markers Within the Legume Family for Germplasm Characterization and Evaluation

Authors
item Wang, Ming
item Gillaspie, Athey
item Harrison Dunn, Melanie
item Dean, Rob - UNIVERSITY OF GA
item Pittman, Roy
item Morris, John
item Pederson, Gary

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: September 22, 2003
Publication Date: January 10, 2004
Citation: Wang, M.L., Gillaspie Jr, A.G., Newman, M.L. aka Harrison Dunn, M.L., Dean, R., Pittman, R.N., Morris, J.B., Pederson, G.A. 2004. Shuffling dna markers within the legume family for germplasm characterization and evaluation. Plant and Animal Genome Conference. Plant and Animal Genome XII, Jan 10-14, 2004, P-530, P. 203

Technical Abstract: Legumes are the third largest flowering plant family with over 700 genera and 20,000 species. Many legumes (including peanut, clover, pigeon pea, cowpea, mung bean, lablab, guar, and others) are maintained at the Plant Genetic Resources Conservation Unit, Griffin, GA. For efficient utilization, legume accessions need to be properly characterized and evaluated. The biggest challenge for molecular characterization and evaluation of plant genetic resources is a lack of adequate DNA markers. Due to limited numbers of DNA markers at present, the legume germplasm is usually described solely by taxonomic and agronomic characters. This greatly hinders current research work and could slow utilization of legume germplasm in the future. In the present studies, Simple Sequence Repeats (SSRs) were collected from existing databases of different legumes (including Medicago, soybean, cowpea, and peanut) and then were used for screening selected accessions from our legume collections. We found that one third of the SSR primers we screened produced amplicons from across-species as well as across-genera. Some of the polymorphic markers have been confirmed and sized on Beckman CEQ8000. Shuffling SSR markers can be a very efficient approach for DNA marker devlopment, especially for minor crops. These polymorphic DNA markers are now being used for characterization and evaluation of our germplasm.

Last Modified: 7/22/2014
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