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United States Department of Agriculture

Agricultural Research Service

Title: Ethylene-Sensitive and Insensitive Regulation of Transcription Factor Expression During in-Vitro Tomato Sepal Ripening

Authors
item Bartley, Glenn
item Ishida, Betty

Submitted to: Journal of Experimental Botany
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 15, 2007
Publication Date: April 23, 2007
Citation: Bartley, G.E., Ishida, B.K. 2007. Ethylene-sensitive and insensitive regulation of transcription factor expression during in-vitro tomato sepal ripening. Journal of Experimental Botany. 58(8):2043-2051.

Interpretive Summary: Ethylene regulation of a number of regulatory factors during cool-temperature induced sepal morphogenesis was revealed. Tomato sepals, when cultured in vitro between 16 and 22 degrees C, appear to change their genetic program to that of ripening fruit. We used 1-methylcyclopropene (1-MCP), to block ethylene induced gene expression by interrupting the ethylene signal transduction pathway that occurs in ripening tomato fruits and possibly in ripening sepals. Regulatory factors previously shown to be up-regulated during cool-temperature induced sepal morphogenesis were not induced in 1-MCP treated sepals indicating ethylene regulation of these transcripts.

Technical Abstract: Ethylene regulation of a number of putative transcription factors during cool-temperature induced sepal morphogenesis was revealed. Lycopersicon esculentum c.v. VFNT Cherry sepals, when cultured in vitro between 16 and 22 degrees C, appear to change their genetic program to that of ripening fruit. We used 1-methylcyclopropene (1-MCP), to block ethylene induced gene expression by interrupting the ethylene signal transduction pathway that occurs in ripening tomato fruits and possibly in ripening sepals. Putative transcription factors previously shown to be up-regulated during cool-temperature induced sepal morphogenesis were not induced in 1-MCP treated sepals indicating ethylene regulation of these transcripts.

Last Modified: 12/21/2014
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