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United States Department of Agriculture

Agricultural Research Service

Title: Intermittent Administration of a Probiotic Culture and a Mixture of Organic Acids May Reduce Salmonella Enteritidis Transmission in Chicks

Authors
item Jarquin, R - UNIV OF ARKANSAS
item Nava, G - UNIV OF ARKANSAS
item Vincente, J - UNIV OF ARKANSAS
item Wolfenden, A - UNIV OF ARKANSAS
item Sartor, C - UNIV OF ARKANSAS
item Higgins, S - UNIV OF ARKANSAS
item Tellez, G - UNIV OF ARKANSAS
item Donoghue, D - UNIV OF ARKANSAS
item Donoghue, Ann
item Hargis, B - UNIV OF ARKANSAS

Submitted to: Southern Poultry Science Society Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: December 10, 2003
Publication Date: September 29, 2004
Citation: Jarquin, R.L., Nava, G.M., Vincente, J.S., Wolfenden, A.D., Sartor, C.D., Higgins, S.E., Tellez, G.I., Donoghue, D.J., Donoghue, A.M., Hargis, B.M. 2004. Intermittent administration of a probiotic culture and a mixture of organic acids may reduce salmonella enteritidis transmission in chicks[abstract]. Poultry Science. 83(Suppl 1):1771.

Technical Abstract: Recently, we have explored the possible inhibitory effect of organic acids on microbial growth within the chicken gastrointestinal tract. The objective of this study was to combine and evaluate a defined probiotic culture and an organic acid mixture on Salmonella colonization in chicks. In three replicate experiments an organic acid mixture (OAM) (tannic , lactic, butyric, acetic, .024%, .048%, .048% and .048% final concentration, respectively) administered in the drinking water was alternated with a nine-bacteria probiotic culture (BB) and evaluated for ability to prevent Salmonella enteritidis (SE) colonization in neonatal broiler chicks. Day-of-hatch chicks were randomly divided into eight pens (n=40/pen). In an additional pen containing 80 chicks, all birds were individually tagged for identification and then orally challenged with SE (10^5) to serve as seeders. Treatments consisted of Controls, OAM alone, BB alone (1 x 10^6 cfu/ml final concentration) or OAM and BB provided on an alternating daily basis (2 pens/treatment). On day 2, 10 seeders were placed into each of the eight pens. Treatments were continued until day 8. On day 10 and 20 (experiment 2 and 3 only) half of the chicks from each pen and the seeders were humanly killed and the cecal tonsils were aseptically collected for enrichment culture for SE. In exp. 1 on Day 10, only the OAM and BB combined treatment resulted in a lower incidence (70%) of SE (p<. 05) compared to Control (95% incidence). In exp. 2 , a significant reduction of SE colonization was observed for all treatment groups compared to control (Control 95%, 75%, OAM alone 35%, 10%, BB alone 55%, 25% and 75%15% for the OAM-BB combination, days 10 and 20 respectively). In exp.3 on day 10, SE incidence was lower (p<. 05) in the OAM group (20%) and the OAM-BB group (42.5%) compared to the control group (72.5%). On day 20, a significant reduction in SE was observed only in the OAM group (47.5%) compared to control (62.5%). These observations show that the combined administration of beneficial bacteria and OAM might be efficacious to prevent SE intestinal colonization however, our results varied considerable in efficacy across experiments.

Last Modified: 8/2/2014
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