Submitted to: Feedinfo.com
Publication Type: Trade Journal
Publication Acceptance Date: December 12, 2003
Publication Date: December 12, 2003
Citation: REGISTER, K.B. DNA-BASED TOOLS FOR IDENTIFICATION OF P. MULTOCIDA AND B. BRONCHISEPTICA. FEEDINFO.COM. 2003. Technical Abstract: Pasteurella multocida and Bordetella bronchiseptica are significant swine respiratory disease pathogens. Traditionally, a definitive diagnosis of infection with P. multocida or B. bronchiseptica is established from clincal signs and isolation of these agents from nasal swabs or biopsy specimens. This approach requires skill and training in the recognition of suspect colonies, is cumbersome, and suffers from a lack of sensitivity. This article discusses colony lift/hybridization and PCR assays presently being used for identification of P. multocida and B. bronchiseptica at the National Animal Disease Center. These assays provide superior sensitivity, are highly specific, require less turn-around time, and are amenable to the processing of multiple samples. A multiplex PCR is described that identifies both B. bronchiseptica and P. multocida, and which also distinguishes toxigenic from nontoxigenic P. multocida. This assay may be especially useful in a diagnostic laboratory setting.