ARS | Publication request: MOLECULAR MAPPING OF A MAJOR GENE FOR GLANDLESS COTTONSEED

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: July 31, 1999
Publication Date: October 31, 1999
Citation: YU, J., KOHEL, R.J. MOLECULAR MAPPING OF A MAJOR GENE FOR GLANDLESS COTTONSEED. AMERICAN SOCIETY OF AGRONOMY MEETING. 1999. ABSTRACT P. 155.

Technical Abstract: Cottonseed is one of the major oilseeds as well as an excellent sdource of protein and feed in the world. The primary quality disadvantage of regular cottonseed is a high level of the dark-colored glands containing toxic gossypol. A major co-dominant gene, G1(@)(^e), was introduced from an egyptian cotton "Bahtim 110" to Texas Marker No. 1(TM-1). Cottons with this gene produce no glands containing gossypol. By use of a cross between a pair of nearly isogenic lines (NILs) differing in GI(@2)(^e), linkage between this gene and DNA markers on chromosome 12 has been established via F(@2) bulked segregants analysis (BSA). A cotton bacterial artificial chromosome (BAC) library is being screened wit the closest markers. The ends of positive BAC clones are used to general more markers around the G1(@)(^e) locus. Tightly linked DNA markers would lead to eventual cloning of the G1(@2)(^e) gene to eliminate gossypol in cottonseed, while retaining glands in other parts of the cotton plants.