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United States Department of Agriculture

Agricultural Research Service

Title: Establishment of Short-Term Cd4+ T Cell Lines Recognizing Neospora Caninum Antigen

Authors
item Tuo, Wenbin
item Davis, W - WASHINGTON STATE PULLMAN
item Fetterer, Raymond
item Jenkins, Mark
item Boyd, Patricia
item Gasbarre, L - USDA ARS BELTSVILLE MD
item Dubey, Jitender

Submitted to: Parasite Immunology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 3, 2004
Publication Date: March 8, 2003
Citation: Tuo, W., Davis, W., Fetterer, R.H., Jenkins, M.C., Boyd, P., Gasbarre, L.C., Dubey, J.P. 2003. Establishment of short-term cd4+ T cell lines recognizing Neospora caninum antigen. Parasite Immunology. 26:243-246.

Interpretive Summary: Neosporosis causes pregnancy loss in cattle. The presence of N. caninum-specific antibodies in circulation may not necessarily provide protection, suggesting neosporosis requires both cell-mediated and humoral immune response for effective protection. The objective of this study was to establish short-term CD4+ T cell lines from peripheral blood mononuclear cells of N. caninum-infected cows. These CD4+ T cells will be used to identify immunodominant Neospora antigens for the development of neosporosis vaccine. Crude N. caninum tachyzoite antigen was prepared from in vitro derived Neospora tachyzoites. Eight of 13 Neospora antigen immunized and N. caninum challenged cows exhibited high levels of proliferation and interferon-gamma production by PBMC in response to antigen stimulation and therefore, some of these cows were used as blood donors to establish CD4+ T cell lines. Multiple Neospora antigen-specific CD4+ T cell lines were established and maintained continuously for 11 weeks in culture by weekly re-stimulation with Neospora tachyzoite antigen and antigen-presenting cells. All cell lines responded highly to antigen stimulation between weeks 3 and 11. Flow cytometric analysis showed that these T cells were composed of primarily CD4+ T cells between week 2 and 8 with a gradually expansion of gamma/delta+ T cells. The results indicate that Neospora antigen-specific CD4+ T cell lines can be established and maintained without the presence of T cell growth factors and may be used to identify immunodominant Neospora antigens. This research will enhance our understanding of bovine cell-mediated immunity to neosporosis and may facilitate development of a proven neosporosis vaccine.

Technical Abstract: Neosporosis causes pregnancy loss in cattle. The presence of N. caninum-specific antibodies in circulation may not necessarily provide protection, suggesting neosporosis requires both cell-mediated and humoral immune response for effective protection. The objective of this study was to establish short-term CD4+ T cell lines from peripheral blood mononuclear cells of N. caninum-infected cows. These CD4+ T cells will be used to identify immunodominant Neospora antigens for the development of neosporosis vaccine. Crude N. caninum tachyzoite antigen was prepared from in vitro derived Neospora tachyzoites. Eight of 13 Neospora antigen immunized and N. caninum challenged cows exhibited high levels of proliferation and interferon-gamma production by PBMC in response to antigen stimulation and therefore, some of these cows were used as blood donors to establish CD4+ T cell lines. Multiple Neospora antigen-specific CD4+ T cell lines were established and maintained continuously for 11 weeks in culture by weekly re-stimulation with Neospora tachyzoite antigen and antigen-presenting cells. All cell lines responded highly to antigen stimulation between weeks 3 and 11. Flow cytometric analysis showed that these T cells were composed of primarily CD4+ T cells between week 2 and 8 with a gradually expansion of gamma/delta+ T cells. The results indicate that Neospora antigen-specific CD4+ T cell lines can be established and maintained without the presence of T cell growth factors and may be used to identify immunodominant Neospora antigens. This research will enhance our understanding of bovine cell-mediated immunity to neosporosis and may facilitate development of a proven neosporosis vaccine.

Last Modified: 7/31/2014
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