Submitted to: Aflatoxin Elimination Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: September 15, 2003
Publication Date: December 15, 2003
Citation: Liang, X.Q., Guo, B.Z., Holbrook, C.C., Lynch, R.E. 2003. Relationship of antifungal protein trypsin inhibitor in peanut resistant and susceptible to Aspergillus flavus [abstract]. In: Proceedings of the 3rd Fungal Genomics, 4th Fumonisin, and 16th Aflatoxin Elimination Workshops, October 13-15, 2003, Savannah, Georgia. p. 58. Technical Abstract: Peanut is frequently subjected to contamination by aflatoxin produced by Aspergillus spp. The objectives of this study were to study the relationship of constitutive expression of trypsin inhibitor (TI) in peanut seeds and resistance to A. flavus infection. The differences of seed infection by A. flavus among 13 genotypes were significant. Yueyou 20 and Zhanque 48 showed high resistant to A. flavus infection. A trypsin inhibitor in peanut seeds was purified by acetone fractionation, followed by passing through DEAE-Sephadex A50 ion-exchange column. The purified inhibitor consisted of two subunits with molecular weight of 10.3 and 17kDs respectively. The inhibition of germination and hypha growth of A. flavus were observed in protein concentration 10'g.ml-1 on V8 juice agar medium. The relationship between the TI concentration/activity and resistant to A. flavus infection was compared. The concentration and activity of TI in resistant genotypes were significantly higher than that in susceptible genotypes.