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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Mapping of the Cotton Genome: Qtl Analysis of Fiber Quality Characteristics

Authors
item Yu, John
item Park, Young-Ha - USDA ARS WESTERN REGION
item Lazo, Gerard - USDA ARS WESTERN REGION
item Kohel, Russell

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: January 18, 1998
Publication Date: January 18, 1998
Citation: YU, J., PARK, Y., LAZO, G.R., KOHEL, R.J. MOLECULAR MAPPING OF THE COTTON GENOME: QTL ANALYSIS OF FIBER QUALITY CHARACTERISTICS. PLANT AND ANIMAL GENOME CONFERENCE. 1998. ABSTRACT P. 352.

Technical Abstract: We have constructed a molecular map in cotton and have identified locations of QTLs for fiber quality properties, by use of F2 progeny from an interspecific cross between two improved cottons, G. hirsutum L. acc. TM-1, and G. barbadense L. acc. 3-79. The unique high quality fiber characteristics of 3-79 and the high productivity and wide adaptability of TM-1 led to our choice of these parents for a polymorphic mapping population. Presented here is a framework map consisting primarily of RFLP and RAPD markers, with some SSR markers, among 171 F2 individuals of TM-1 X 3-79. These 219 loci are assembled into 40 linkage groups and cover 3,855 cM of the cotton genome. About one half of the linkage groups have been assigned to their respective genomic origin (A vs. D) or chromosomal identity (1 through 26) by use of diploid and aneuploid cottons. As bundle fiber strength, fiber length, fiber fineness, and other fiber properties have been observed to display quantitative inheritance, the differences between TM-1 and 3-79 (18.4 vs. 27.4 cN/tex for strength, 1.17 vs. 1.38 inches for length, and 4.49 vs. 3.63 micronaire units for fineness, respectively) facilitates QTL mapping by use of the same 171 F2 individuals. With both MapMaker/QTL and SAS program, we have detected two QTLs for bundle fiber strength (Sf), three QTLs for fiber length (Lf), and five QTLs for fiber fineness (Ff) in different linkage groups. These QTLs explain about 35% to 50% of the total genetic variance for fiber characteristics in the F2 population. Further characterization of these QTLs is underway by use of recombinant inbred lines of the same mapping individuals, and also in the intraspecific G. hirsutum crosses.

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