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United States Department of Agriculture

Agricultural Research Service

Title: Genetically Engineered Escherichia Coli for Ethanol Production from Pentose Sugars: Substrate and Product Inhibition and Kinetic Parameters

Authors
item QURESHI, NASIB
item DIEN, BRUCE
item NICHOLS, NANCY
item COTTA, MICHAEL

Submitted to: Biotechnology for Fuels and Chemicals Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: May 12, 2004
Publication Date: May 12, 2004
Citation: Qureshi, N., Dien, B.S., Nichols, N.N., Cotta, M.A. 2004. Genetically engineered Escherichia coli for ethanol production from pentose sugars: substrate and product inhibition and kinetic parameters [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 5-23.

Technical Abstract: In order to exploit full potential of economically available lignocellulosic biomass, Escherichia coli FBR5, a bacterial strain capable of producing ethanol efficiently in continuous cultures, was studied for substrate and product inhibition. The inhibition studies were performed under batch conditions. The culture can tolerate a maximum xylose concentration of 200 gL**-1; however, with a poor cell growth. The substrate inhibition studies were performed to observe cell growth and ethanol production. Similarly, ethanol inhibition studies were also performed. At an ethanol concentration of 60 gL**-1, no cell growth was observed. Further studies were extended to study the effect of medium component(s) on cell growth and ethanol production. Kinetic parameters (Saturation constant, Ks and Michaelis Menten constant, Km) that are essential for process scale up were measured. These studies were performed in order to be able to integrate fermentation with product recovery. Such a process integration benefits process economics.

Last Modified: 9/10/2014